However, were an average of 1.

Attempt was made to identify groups of bacteria E. coli population in the aerobic treatment plants changed in species composition and whether there were changes in their characteristics. Bacteria were isolated on media with minimal selectivity is based on the utilization of glucose rather than lactose. The spread plate (SP) and membrane filtration (MF) methods were used and compared with intestinal MF methods. To test whether changes in intestinal populations during wastewater treatment, fifty morphological and biochemical tests were conducted on each isolate. The results were analyzed by calculating the Euclidean distance and the use of full respect for the numerical classification for the formation of clusters of isolates with similar characteristics. Clusters were defined by including the reference culture Enterobacteriaceae isolates and identification of selection by API 20E method. From the 857 strains characterized and classified, 369 (43. 1%) were identified as Escherichia coli, Citrobacter, Enterobacter and Klebsiella SPP. SP and MF methods with glucose-containing media (PRGA-SP and TGEA / PRGB-MF) showed fewer false-positive group of bacteria E. coli than the method of intestinal MF using M-Endo-LES agar. The average number of E. coli in raw and treated sewage was 4. 2 x 10 ⁵ / ml and 1. 7 x 10 m ³ / ml, respectively, the number of Citrobacter, Enterobacter arid K1ebsiella SPP. However, were an average of 1. 2 x 10 ⁶ / ml and 1. 0 x 10 ⁴ / ml, respectively. The number of bacteria in clusters, in which E. coli were placed were reduced to 99. 4 - 99. 7% for wastewater treatment, and those in clusters Citrobacter, Enterobacter and Klebsiella SPP. 95. 9-99. 8%. Isolates in some clusters of these four genera have been better to survive the sewage treatment than others. E coli decreased in proportion to the population of intestinal bacteria group. The number of bacteria represented by each group hesitated noticeably over time, indicating the sequence of species and habitats of bacteria in sewage. In most cases bacteria isolated in treated sewage were detected in sewage samples at the same time, suggesting that the removal of bacteria in wastewater treatment are more likely than the growth of certain habitats. Discriminant analysis showed that there were small but significant differences between isolates from raw or treated sewage and those isolated by different methods. Biotype of Escherichia coli, Citrobacter, Enterobacter and Klebsiella SPP. revealed intestinal and fecal intestinal membrane filter methods can be identified most clearly with those isolated PRGA-SP and TGEA / PRGB-MF methods. E. coli was the only species that can be clearly and easily separated from other bacterial species Escherichia coli and other genera using modified IMViC scheme. Other bacteria E. coli can not be separated. Isolates identified as E. coli were placed in strattera online four separate clusters, i. ie, different habitats. It was concluded that, since E. coli can be most easily identified are the most numerous E. coli in stool and is least likely to multiply in natural situations outside of the intestine, it must be true indicator of fecal contamination, even after wastewater treatment. .